Archive Issue Details

Archive Issue Details Volume.2 ,Issue . 3

Manuscript Number
IJPRT-2023-08-027

Manuscript Title
Analytical Method Development and Validation of UHPLC and UV Spectroscopy for the Determination of Obeticholic acid in Bulk and Pharmaceutical Dosage Form

Article Type
Original Research Articles

Author Names

Patil Devyani *
Javesh Patil

Abstract
Quantitative determination of obeticholic acid is simple, quick, sensitive, accurate, precise, and robust. Using a C18 column (4.6mm X 100mm) as the stationary phase, 0.05% OPA (pH 3.7), and Methanol: water (0.05% OPA) 50:50 as the mobile phase, chromatographic separation has been performed at a flow rate of 0.7 ml. The wavelength used for UV detection was 210 nm. Linearity, accuracy, range, and robustness were all within acceptable bounds as per the ICH criteria. The determination of Obeticholi

Keywords .
UHPLC, UV Spectroscopy, Obeticholic acid, validation method

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