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Abstract
The present study attempts to validate the approach by UV-Spectroscopy at a wavelength of 273nm. The UHPLC separation was performed using reversed phase chromatography on a C18 column (100mmX4.6mm, 2.5m) in gradient mode at a flow rate of 1.0 mL min. At a wavelength of 273nm, diode array detection was performed using a mobile phase composed of a 50:50 (v/v) mixture of methanol and water (0.1% acetic acid).The technique for Gemfibrozil was linear in the concentration range of 5 - 25 g/ml, with a

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